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Results: Four additional K Ras mutations Leu19Phe 1 out of 106 tumours, Lys117Asn 1 out of 106, Ala146Thr 7 out of 106 and Arg164Gln 1 out of 106 were identified. Lys117Asn and Ala146Thr had burberry weekend phenotypes similar to the hotspot mutations, whereas Leu19Phe had an attenuated phenotype and the Arg164Gln burberry belts mutation was phenotypically burberry raincoat equivalent to wt K Ras. We additionally identified a new K Ras gene amplification event, present in approximately 2 of tumours. Conclusions: The identification of mutations outwith previously described hotspot codons increases the K Ras mutation burden in colorectal tum


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